4.5 Article

The Establishment of a Fast and Safe Orthotopic Colon Cancer Model Using a Tissue Adhesive Technique

期刊

CANCER RESEARCH AND TREATMENT
卷 53, 期 3, 页码 733-743

出版社

KOREAN CANCER ASSOCIATION
DOI: 10.4143/crt.2020.494

关键词

Orthotopic; Colon neoplasms; Mouse model; Tissue adhesive technique

类别

资金

  1. University of Ulsan, College of Medicine
  2. Asan Institute for Life Sciences [2016 IP0571]

向作者/读者索取更多资源

The study showed that using tissue adhesive to establish a colon cancer model resulted in increased graft success, decreased mortality, larger tumor size, and shorter operation time compared to the conventional surgical method. Additionally, the tissue adhesive group had similar tumor structure, immunohistochemistry staining, and most tumor markers as the suture group, with alterations in apoptosis and proliferation markers observed.
Purpose We aimed to develop a novel method for orthotopic colon cancer model, using tissue adhesive in place of conventional surgical method. Materials and Methods RFP HCT 116 cell line were used to establish the colon cancer model. Fresh tumor tissue harvested from a subcutaneous injection was grafted into twenty nude mice, divided into group A (suture method) and group B (tissue adhesive method). For the group A, we fixed the tissue on the serosa layer of proximal colon by 8-0 surgical suture. For the group B, tissue adhesive (10 & micro;L) was used to fix the tumor. The mortality, tumor implantation success, tumor metastasis, primary tumor size, and operation time were compared between the two groups. Dissected tumor tissue was analyzed for the histology and immunohistochemistry. Also, we performed tumor marker analysis. Results We observed 30% increase in graft success and 20% decrease in mortality, by using tissue adhesive method, respectively. The median colon tumor size was significantly increased by 4 mm and operation time was shortened by 6.5 minutes. The H&E showed similar tumor structure between the two groups. The immunohistochemistry staining for cancer antigen 19-9, carcinoembryonic antigen, cytokeratin 20, and Ki-67 showed comparable intensities in both groups. Real-time quantitative reverse transcription analysis showed eight out of nine tumor markers are unchanged in the tissue adhesive group. Western blot indicated the tissue adhesive group expressed less p-JNK (apototic marker) and more p-MEK/p-p38 (proliferation marker) levels. Conclusion We concluded the tissue adhesive method is a quick and safe way to generate orthotopic, colon cancer model.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据