Promiscuous Installation of D-Amino Acids in Gene-Encoded Peptides

D-Amino acids can have major effects on the structure, proteolytic stability, and bioactivity of peptides. Proteusin radical S-adenosyl methionine epimerases regioselectively install such residues in ribosomal peptides to generate peptides with the largest number of D-residues currently known in biomolecules. To study their utility in synthetic biology, we investigated the substrate tolerance and substrate-product relationships of the cyanobacterial model epimerase OspD using libraries of point mutants as well as distinct extended peptides that were fused to an N-terminal leader sequence. OspD was found to exhibit exceptional substrate promiscuity in E. coli, accepting 15 different amino acids and converting peptides with a broad range of compositions, secondary structures, and polarities. Diverse single and multiple epimerization patterns were identified that were dictated by the peptide sequence. The data suggest major potential in creating genetically encoded products previously inaccessible by synthetic biology.

Automated summary

D-amino acids can significantly affect the structure and bioactivity of peptides. The epimerase OspD shows exceptional substrate promiscuity in E. coli, accepting a wide range of amino acids and converting peptides with diverse compositions, structures, and polarities, suggesting potential for creating genetically encoded products in synthetic biology.