期刊
ACS SYNTHETIC BIOLOGY
卷 10, 期 1, 页码 107-114出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.0c00467
关键词
bacteriophage; transduction; cosmid; bacteriophage therapy; recombineering; cosmid
资金
- Biotechnology and Biological Sciences Research Council [BB/P020615/1, BB/M01116X/1, BB/M017982/1]
- Medical Research Council [1936220]
- Warwick-Monash Alliance
- UK Research and Innovation block grant
- BBSRC [BB/P020615/1, BB/M017982/1] Funding Source: UKRI
- MRC [1936220] Funding Source: UKRI
This study presents a method to produce transducing particles without contamination and demonstrates their use as an antimicrobial agent. This paves the way for new phage-based technologies with greater scope than lytic phage therapy.
Natural and engineered phages have been used in many applications, but their use to deliver user-defined genetic cargoes has been hampered by contamination with replicative phage, restricting use of the technology beyond the laboratory. Here we present a method to produce transducing particles without contamination. In addition, we demonstrate the use of a helper phage-free transducing particle preparation as an antimicrobial agent. This will pave the way for the development of new phage-based technologies with greater scope than lytic phage therapy.
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