Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 mu g cm(-2) nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.

Automated summary

The study embedded an antimicrobial peptide, Nisin Z, within polyelectrolyte multilayers composed of natural polysaccharides to create a multilayer with antimicrobial properties. The peptide-containing films showed excellent antimicrobial efficacy against Staphylococcus aureus and MRSA strains, unaffected by its location within the multilayer, demonstrating the potential for creating functionalized coatings in industries such as food, biomaterials, and pharmaceuticals.