期刊
SCIENTIFIC REPORTS
卷 10, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-020-77786-5
关键词
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资金
- Novo Nordisk Postdoctoral Fellowships
- Wellcome trust [WT106174/Z/14/Z]
- National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) PhD Studentship [NC/S001689/1]
- National Institutes of Health (NIH) [R01 AA0278707]
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/I021086/1, BB/S015817/1]
- University of Oxford
- BBSRC [BB/S015817/1, BB/I021086/1] Funding Source: UKRI
Body temperature is an important physiological parameter in many studies of laboratory mice. Continuous assessment of body temperature has traditionally required surgical implantation of a telemeter, but this invasive procedure adversely impacts animal welfare. Near-infrared thermography provides a non-invasive alternative by continuously measuring the highest temperature on the outside of the body (T-skin), but the reliability of these recordings as a proxy for continuous core body temperature (T-core) measurements has not been assessed. Here, T-core (30 s resolution) and T-skin (1 s resolution) were continuously measured for three days in mice exposed to ad libitum and restricted feeding conditions. We subsequently developed an algorithm that optimised the reliability of a T-skin-derived estimate of T-core. This identified the average of the maximum T-skin per minute over a 30-min interval as the optimal way to estimate T-core. Subsequent validation analyses did however demonstrate that this T-skin-derived proxy did not provide a reliable estimate of the absolute T-core due to the high between-animal variability in the relationship between T-skin and T-core. Conversely, validation showed that T-skin-derived estimates of T-core reliably describe temporal patterns in physiologically-relevant T-core changes and provide an excellent measure to perform within-animal comparisons of relative changes in T-core.
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