Transcript levels of keratin 1/5/6/14/15/16/17 as potential prognostic indicators in melanoma patients

Keratins (KRTs), the intermediate filament-forming proteins of epithelial cells, are extensively used as diagnostic biomarkers in cancers and associated with tumorigenesis and metastasis in multiple cancers. However, the diverse expression patterns and prognostic values of KRTs in melanoma have yet to be elucidated. In the current study, we examined the transcriptional and clinical data of KRTs in patients with melanoma from GEO, TCGA, ONCOMINE, GEPIA, cBioPortal, TIMER and TISIDB databases. We found that the mRNA levels of KRT1/2/5/6/8/10/14/15/16/17 were significantly differential expressed between primary melanoma and metastatic melanoma. The expression levels of KRT1/2/5/6/10/14/15/16/17 were correlated with advanced tumor stage. Survival analysis revealed that the high transcription levels of KRT1/5/6/14/15/16/17 were associated with low overall survival in melanoma patients. GSEA analysis indicated that the most involved hallmarks pathways were P53 pathway, KRAS signaling, estrogen response early and estrogen response late. Furthermore, we found some correlations among the expression of KRTs and the infiltration of immune cells. Our study may provide novel insights for the selection of prognostic biomarkers for melanoma.

Automated summary

In this study, the diverse expression patterns and prognostic values of keratins (KRTs) in melanoma were investigated, revealing differential expression of KRT1/2/5/6/10/14/15/16/17 between primary and metastatic melanoma and their correlation with advanced tumor stage and overall survival in patients. Pathway analysis identified involvement of P53 pathway, KRAS signaling, estrogen response early and late in melanoma, along with correlations between KRT expression and immune cell infiltration.