期刊
NUTRIENTS
卷 13, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/nu13010123
关键词
pro-inflammatory cytokine; interleukin; gene expression; transepithelial electrical resistance; permeability
资金
- University of Warmia and Mazury grants
- Faculty of Biology and Biotechnology
Osthole has anti-inflammatory potential in LPS-induced cell inflammatory reactions, reducing cytokine secretion and affecting key gene expressions in a concentration-dependent manner. However, it also increases cell monolayer permeability, so its safety as a potential drug for intestinal inflammatory symptoms should be further confirmed.
Lipopolysaccharydes (LPS) are responsible for the intestinal inflammatory reaction, as they may disrupt tight junctions and induce cytokines (CKs) secretion. Osthole has a wide spectrum of pharmacological effects, thus its anti-inflammatory potential in the LPS-treated Caco-2 cell line as well as in Caco-2/THP-1 and Caco-2/macrophages co-cultures was investigated. In brief, Caco-2 cells and co-cultures were incubated with LPS to induce an inflammatory reaction, after which osthole (150-450 ng/mL) was applied to reduce this effect. After 24 h, the level of secreted CKs and changes in gene expression were examined. LPS significantly increased the levels of IL-1 beta, -6, -8, and TNF-alpha, while osthole reduced this effect in a concentration-dependent manner, with the most significant decrease when a 450 ng/mL dose was applied (p < 0.0001). A similar trend was observed in changes in gene expression, with the significant osthole efficiency at a concentration of 450 ng/mu L for IL1R1 and COX-2 (p < 0.01) and 300 ng/mu L for NF-kappa B (p < 0.001). Osthole increased Caco-2 monolayer permeability, thus if it would ever be considered as a potential drug for minimizing intestinal inflammatory symptoms, its safety should be confirmed in extended in vitro and in vivo studies.
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