期刊
NUTRIENTS
卷 12, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/nu12123697
关键词
DNA methylation; epigenetics; Adventist Health Study-2; vegetarian diet; linear regression; permutation
资金
- Ardmore Institute of Health
- National Institute of Health (National Cancer Institute) [R01 CA094594]
- National Institute of Health (National Institute of Aging) [1R01AG026348]
- Loma Linda University [2120211, 2130279, 2170322]
We sought to determine if DNA methylation patterns differed between vegans and non-vegetarians in the Adventist Health Study-2 cohort. Genome-wide DNA methylation derived from buffy coat was profiled in 62 vegans and 142 non-vegetarians. Using linear regression, methylation of CpG sites and genes was categorized or summarized according to various genic/intergenic regions and CpG island-related regions, as well as the promoter. Methylation of genes was measured as the average methylation of available CpG's annotated to the nominated region of the respective gene. A permutation method defining the null distribution adapted from Storey et al. was used to adjust for false discovery. Differences in methylation of several CpG sites and genes were detected at a false discovery rate < 0.05 in region-specific and overall analyses. A vegan diet was associated predominantly with hypomethylation of genes, most notably methyltransferase-like 1 (METTL1). Although a limited number of differentially methylated features were detected in the current study, the false discovery method revealed that a much larger proportion of differentially methylated genes and sites exist, and could be detected with a larger sample size. Our findings suggest modest differences in DNA methylation in vegans and non-vegetarians, with a much greater number of detectable significant differences expected with a larger sample.
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