期刊
MATERIALS
卷 14, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/ma14010223
关键词
tooth-derived scaffold; demineralized dentin matrix; bio-absorption; bone regeneration
类别
资金
- HOYA Technosurgical Corporation
- Strategic Promotion of Innovative Research and Development, Japan Science and Technology Agency
The study evaluated the bio-absorption and bone regeneration of human tooth-derived dentin scaffold, PR-DDM, in critical-size iliac defects after in vivo implantation. The morphological changes due to bio-absorption by the cellular phagocytes were comparable to the 3D micro-CT and histological images at 2 and 4 months, demonstrating that the PR-DDM block was gradually absorbed by multinucleated giant cells and regenerated bone. PR-DDM may serve as a unique scaffold for extraoral bone regeneration.
The aim of this study was to evaluate the bio-absorption and bone regeneration of human tooth-derived dentin scaffold, entitled as perforated root-demineralized dentin matrix (PR-DDM), after in vivo implantation into the critical-size iliac defects. The dentin scaffolds were prepared from human vital, non-functional teeth. Thirty artificial macro-pores (o 1 mm) were added after removing the enamel portion. The modified teeth were supersonically demineralized in 0.34 N HNO3 for 30 min. The microstructure was observed by scanning electron microscope (SEM). The 3D micro-CT and histological analysis were carried out to evaluate the bio-absorption of PR-DDM at 2 and 4 months. A smooth dentin collagen surface with symmetrical macro-pores and tube-type dentinal tubules (o 1-2 mu m) with micro-cracks were observed on the perforated region. A significant number of custom-made macro-pores disappeared, and the size of the macro-pores became significantly wider at 4 months compared with the 2 months (p < 0.05) evaluated by 3D micro-CT. Histological images revealed the presence of multinucleated giant cells attached to the scalloped border of the PR-DDM. The morphological changes due to bio-absorption by the cellular phagocytes were comparable to the 3D micro-CT and histological images at 2 and 4 months. Altogether, the results demonstrated that the PR-DDM block was gradually absorbed by multinucleated giant cells and regenerated bone. Human PR-DDM might serve as a unique scaffold for extraoral bone regeneration.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据