期刊
JOURNAL OF OVARIAN RESEARCH
卷 14, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13048-020-00742-4
关键词
Apoptosis; ceRNA; Polycystic ovarian syndrome; Proliferation
资金
- TCM science and technology development plan project of Shandong Province [2019-0888]
- Natural Science Foundation of Anhui Province [KJ2018A0691]
- Project of Cultivating Outstanding Talents in Colleges [gxgwfx2019072]
In patients with PCOS, the expression of HOTAIRM1 and PIK3CD is upregulated, while miR-433-5p is downregulated. HOTAIRM1 promotes the expression of PIK3CD, affecting the proliferation and apoptosis of ovarian granulosa cells. miR-433-5p interacts with PIK3CD, modulating the function of granulosa cells.
BackgroundCurrently, several non-coding RNAs (ncRNAs) were distinguished in polycystic ovarian syndrome (PCOS). This present study aims to explore the potential function of lncRNA HOTAIRM1/miR-433-5p/PIK3CD in ovarian granulosa cells.MethodsWe analyzed the expression profiles of HOTAIRM1, miR-433-5p and PIK3CD in PCOS samples by enquiring GEO database. GSEA was applied to enrich the pathways related to PCOS. The target association between HOTAIRM1 and miR-433-5p or the binding association between miR-433-5p and PIK3CD were assessed by online prediction tools and a dual luciferase reporter assay. qPCR and western blotting assays were used to detect PIK3CD expression after HOTAIRM1 and miR-433-5p treatment. The proliferation and apoptosis of ovarian granulosa cells were estimated by cell counting kit-8 and flow cytometry assays, respectively.ResultsThe expression profiles of HOTAIRM1 and PIK3CD were increased, whereas miR-433-5p was decreased in PCOS tissues. PIK3CD expression was positively regulated by HOTAIRM1 and negatively modulated by miR-433-5p. Overexpression of HOTAIRM1 reduced the proliferative ability and increased the apoptotic ability of granulosa cells, whereas upregulation of miR-433-5p or downregulation of PIK3CD reversed the effects of HOTAIRM1 on granulosa cells. Moreover, overexpression of miR-433-5 displayed a results with increasing proliferative ability and decreasing apoptotic ability, but upregulation of PIK3CD eliminated the function of miR-433-5p on granulosa cells.ConclusionsOur findings illustrated that HOTAIRM1 could sponge miR-433-5p to promote PIK3CD expression, thereby regulating the growth and apoptosis of granulose cells in PCOS.
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