期刊
BIOMEDICAL OPTICS EXPRESS
卷 12, 期 1, 页码 303-319出版社
Optica Publishing Group
DOI: 10.1364/BOE.411888
关键词
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资金
- National Science Foundation [NSF ECCS-1846659]
- Air Force Office of Scientific Research [FA9550-19-1-0323]
- National Institutes of Health [NIGMS R35 GM133616]
- Australian National Fabrication Facility
- CSIRO ECR (CERC) Program
- ECR Grant travel award
- Burroughs Wellcome Fund Career Award
- Boston University Cross-Disciplinary PhD Fellowship
- Nanotechnology Innovation Center, Boston University
- CSIRO Research+ Science Leader Progran
Label-free vibrational imaging using VIPPS technology provides chemical identification and high contrast imaging of biological samples, showing potential for imaging cell models and tissues without the need for labeling. This technique combines absorption signatures and sensitive heat detection to enhance detectability of nano-sized structures, offering a novel approach to studying intracellular dynamics.
Label-free vibrational imaging of biological samples has attracted significant interest due to its integration of structural and chemical information. Vibrational infrared photothermal amplitude and phase signal (VIPPS) imaging provide label-free chemical identification by targeting the characteristic resonances of biological compounds that are present in the midinfrared fingerprint region (3 mu m - 12 mu m). High contrast imaging of subcellular features and chemical identification of protein secondary structures in unlabeled and labeled fibroblast cells embedded in a collagen-rich extracellular matrix is demonstrated by combining contrast from absorption signatures (amplitude signals) with sensitive detection of different heat properties (lock-in phase signals). We present that the detectability of nano-sized cell membranes is enhanced to well below the optical diffraction limit since the membranes are found to act as thermal barriers. VIPPS offers a novel combination of chemical imaging and thermal diffusion characterization that paves the way towards label-free imaging of cell models and tissues as well as the study of intracellular heat dynamics. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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