期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-020-20869-8
关键词
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资金
- National Natural Science Foundation of China [21522505, U19A2005]
- Multi-Disciplinary Research Program of Sichuan University [2020KXK0404]
- Fundamental Research Funds for Central Universities [2018SCUH0075]
- public Platform of Analytical and Testing Center, Sichuan University
Researchers have developed a photosensitizer that can work in extremely acidic environments to combat aciduric bacteria, demonstrating inhibition of bacterial growth in acidic beverages and successful photodynamic inactivation of Candidiasis.
Aciduric bacteria that can survive in extremely acidic conditions (pH < 4.0) are challenging to the current antimicrobial approaches, including antibiotics and photodynamic bacteria inactivation (PDI). Here, we communicate a photosensitizer design concept of halogenation of fluorescein for extremely acidic PDI. Upon halogenation, the well-known spirocyclization that controls the absorption of fluorescein shifts to the acidic pH range. Meanwhile, the heavy atom effect of halogens boosts the generation of singlet oxygen. Accordingly, several photosensitizers that could work at even pH < 2.0 were discovered for a broad band of aciduric bacteria families, with half maximal inhibitory concentrations (IC50) lower than 1.1 mu M. Since one of the discovered photosensitizers is an FDA-approved food additive (2',4',5',7'-tetraiodofluorescein, TIF), successful bacteria growth inhibition in acidic beverages was demonstrated, with greatly extended shelf life from 2 days to similar to 15 days. Besides, the in vivo PDI of Candidiasis with TIF under extremely acidic condition was also demonstrated. Photodynamic inactivation is a common antibacterial method but often fails in extremely acidic environments (pH< 4). Here, the authors demonstrate halogenation of fluorescein for the development of a photodynamic agent which works in extremely acidic environments and demonstrate sterilisation applications in fruit juice and in vivo.
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