4.8 Article

Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants

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NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -

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NATURE PORTFOLIO
DOI: 10.1038/s41467-020-20728-6

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  1. Austrian Science Fund [SFB F34, I 3685-B25, I1468, AI02955, AI03685, W1207-B09]
  2. Austrian Science Fund (FWF) [I1468] Funding Source: Austrian Science Fund (FWF)

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Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). Using a combination of long- and short-read sequencing, researchers have identified tissue-specific variants of rRNA genes integrated into translating ribosomes in Arabidopsis NOR2 rDNA.
Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). The NOR is constituted of multiple, highly repetitive rDNA genes, encoding the catalytic ribosomal core RNAs which are transcribed from 45S rDNA units. Their precise sequence information and organization remain uncharacterized. Here, using a combination of long- and short-read sequencing technologies we assemble contigs of the Arabidopsis NOR2 rDNA domain. We identify several expressed rRNA gene variants which are integrated into translating ribosomes in a tissue-specific manner. These findings support the concept of tissue specific ribosome subpopulations that differ in their rRNA composition and provide insights into the higher order organization of NOR2. The nucleolus organizing region (NOR) consists of multiple, highly repetitive rDNA genes. Here Sims et al. use both long- and short-read sequencing to determine the organization and sequence of Arabidopsis NOR2 rDNA and show that different rRNA gene variants are integrated into translating ribosomes in a tissue-specific manner.

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