期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-020-19827-1
关键词
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资金
- Aker Scholarship
- NanoMed Marie Skodowska-Curie ITN from the H2020 programme [676137]
- H2020 through the Individual Marie Skodowska-Curie Fellowship IMAGINE [701713]
- Arthritis Research U.K. [21138]
- Medical Research Council [MR/S00551X/1]
- European Research Council (ERC) Seventh Framework Programme Consolidator grant Naturale CG [616417]
- European Union's Horizon 2020 research and innovation programme under the Marie Skodowska-Curie Individual European Fellowship [746980]
- Wellcome Trust Senior Investigator Award [098411/Z/12/Z]
- European Research Council Consolidator Grant [772853 - ENTRAPMENT]
- Wellcome Trust Senior Research Fellowship [206444/Z/17/Z]
- Lister Institute of Preventive Medicine
- H2020 through the Individual Marie Skodowska-Curie Fellowship MYCO TRAPS [700088]
- Marie Curie Actions (MSCA) [701713, 700088, 746980, 676137] Funding Source: Marie Curie Actions (MSCA)
- MRC [MR/S00551X/1] Funding Source: UKRI
Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and Delta RD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos. Raman spectroscopic imaging (RSI) can provide information on the chemical composition of a sample, but application to living organisms has lacked sufficient spatial resolution and signal strength. Here the authors apply confocal RSI to whole-mount zebrafish embryos to distinguish different infectious bacteria and to living zebrafish embryos to monitor the wound healing process.
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