期刊
NATURE COMMUNICATIONS
卷 11, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41467-020-19876-6
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资金
- International Max Planck Research School for Molecular Life Sciences (IMPRS-LS)
- Deutsche Forschungsgemeinschaft [GRK1657/TP1C, CA198/9-2]
- DFG [SPP 2202]
- National Human Genome Research Institute [RM1-HG007743-02CEGS]
- NSF Physics Frontiers Center Award [PHY1427654]
- Welch Foundation [Q-1866]
- USDA Agriculture and Food Research Initiative Grant [2017-05741]
- NIH 4D Nucleome Grant [U01HL130010]
- NIH Encyclopedia of DNA Elements Mapping Center Award [UM1HG009375]
Cohesin plays an essential role in chromatin loop extrusion, but its impact on a compartmentalized nuclear architecture, linked to nuclear functions, is less well understood. Using live-cell and super-resolved 3D microscopy, here we find that cohesin depletion in a human colon cancer derived cell line results in endomitosis and a single multilobulated nucleus with chromosome territories pervaded by interchromatin channels. Chromosome territories contain chromatin domain clusters with a zonal organization of repressed chromatin domains in the interior and transcriptionally competent domains located at the periphery. These clusters form microscopically defined, active and inactive compartments, which likely correspond to A/B compartments, which are detected with ensemble Hi-C. Splicing speckles are observed nearby within the lining channel system. We further observe that the multilobulated nuclei, despite continuous absence of cohesin, pass through S-phase with typical spatio-temporal patterns of replication domains. Evidence for structural changes of these domains compared to controls suggests that cohesin is required for their full integrity.
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