期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE RESEARCH
DOI: 10.1038/s41467-020-20556-8
关键词
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资金
- Danish Council for Independent Research Medical Sciences [FSS 6110-00498B/FNU 8021-00072B]
- Novo Nordisk Foundation [NNF16OC0023046, NNF17OC0027224, NNF15CC0001]
- Lundbeck Foundation [R266-2017-4358]
- research program (2016) Physical Activity and Nutrition for Improvement of Health - University of Copenhagen
- Danish Diabetes Academy - Novo Nordisk Foundation
- TrygFonden
- Danish National Research Foundation [DNRF55]
- Novo Nordisk Foundation Center [NNF18CC0034900]
The study developed a workflow for proteomics analysis of isolated muscle fibers from freeze-dried human muscle biopsies, identifying over 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training altered expression of proteins involved in various metabolic pathways in a fiber type-specific manner, providing insights into molecular mechanisms underlying muscle function and health.
Skeletal muscle conveys several of the health-promoting effects of exercise; yet the underlying mechanisms are not fully elucidated. Studying skeletal muscle is challenging due to its different fiber types and the presence of non-muscle cells. This can be circumvented by isolation of single muscle fibers. Here, we develop a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies. We identify more than 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training alters expression of 237 and 172 proteins in slow- and fast-twitch muscle fibers, respectively. Interestingly, expression levels of secreted proteins and proteins involved in transcription, mitochondrial metabolism, Ca2+ signaling, and fat and glucose metabolism adapts to training in a fiber type-specific manner. Our data provide a resource to elucidate molecular mechanisms underlying muscle function and health, and our workflow allows fiber type-specific proteomic analyses of snap-frozen non-embedded human muscle biopsies.
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