期刊
EPIGENOMICS
卷 13, 期 3, 页码 187-202出版社
FUTURE MEDICINE LTD
DOI: 10.2217/epi-2020-0353
关键词
autophagy; bioinformatics; diabetic nephropathy; epigenetics; flavonoid plants; isorhamnetin; miRNA; rtPCR; RNA; western blot
资金
- scientific and technology development foundation (STDF), Egypt [22907]
The study demonstrated the significant nephroprotective effect of isorhamnetin in a Type 2 diabetes mellitus rat model, showing improvements in blood glucose and lipid profiles, as well as modulation of autophagy-related genes and miRNA regulation.
Aim: To assess isorhamnetin efficacy for diabetic kidney disease in a Type 2 diabetes mellitus rat model, through investigating its effect at the epigenetic, mRNA and protein levels. Materials & methods: Type 2 diabetes mellitus was induced in rats by streptozotocin and high-fat diet. Rats were treated with isorhamnetin (50 mg/kg/d) for 4 or 8 weeks. Fasting blood glucose, renal and lipid profiles were evaluated. Renal tissues were examined by light and electron microscopy. Autophagy genes (FYCO1, ULK, TECPR1 and WIPI2) and miR-15b, miR-34a and miR-633 were assessed by qRT-PCR, and LC3A/B by immunoblotting. Results: Isorhamnetin improved fasting blood glucose, renal and lipid profiles with increased autophagosomes in renal tissues. It suppressed miRNA regulation of autophagy genes Conclusion: We propose a molecular mechanism for the isorhamnetin renoprotective effect by modulation of autophagy epigenetic regulators.
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