期刊
BMC OPHTHALMOLOGY
卷 20, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12886-020-01751-z
关键词
Scleral cross-linking; Rabbit sclera; Glutaraldehyde; Paraformaldehyde; Riboflavin; UV-A-light; Riboflavin; blue light
资金
- German Federal Ministry of Education and Research (BMBF) [1315883]
- Deutsche Forschungsgemeinschaft [FR 1825/1-1]
- Government of Saxony, Germany (Ministry for Science and Art and Sachsische Aufbau-Bank) [100 175 031, 5081-7008]
- Projekt DEAL
Background Collagen cross-linking of the sclera is a promising approach to strengthen scleral rigidity and thus to inhibit eye growth in progressive myopia. Additionally, cross-linking might inhibit degrading processes in idiopathic melting or in ocular inflammatory diseases of the sclera. Different cross-linking treatments were tested to increase resistance to enzymatic degradation of the rabbit sclera. Methods Scleral patches from rabbit eyes were cross-linked using paraformaldehyde, glutaraldehyde or riboflavin combined with UV-A-light or with blue light. The patches were incubated with collagenase I (MMP1) for various durations up to 24 h to elucidate differences in scleral resistance to enzymatic degradation. Degraded protein components in the supernatant were detected and quantified using measurements of Fluoraldehyde o-Phthaldialdehyde (OPA) fluorescence. Results All cross-linking methods reduced the enzymatic degradation of rabbit scleral tissue by MMP1. Incubation with glutaraldehyde (1%) and paraformaldehyde (4%) caused nearly a complete inhibition of enzymatic degradation (down to 7% +/- 2.8 of digested protein compared to control). Cross-linking with riboflavin/UV-A-light reduced the degradation by MMP1 to 62% +/- 12.7 after 24 h. Cross-linking with riboflavin/blue light reduced the degradation by MMP1 to 77% +/- 13.5 after 24 h. No significant differences could be detected comparing different light intensities, light exposure times or riboflavin concentrations. Conclusions The application of all cross-linking methods increased the resistance of rabbit scleral tissue to MMP1-degradation. Especially, gentle cross-linking with riboflavin and UV-A or blue light might be a clinical approach in future.
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