期刊
JOURNAL OF FUNCTIONAL FOODS
卷 75, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jff.2020.104217
关键词
Lipophilic grape seed proanthocyanidin; Anti-inflammatory; RAW 264.7 cells; Zebrafish
资金
- foundation of Key Laboratory of Tropical Crop Products Processing of Ministry of Agriculture and Rural Affairs, P.R. China [KFKT201901]
- Natural Science Foundation of Jiangxi Province [20202BAB215014]
Grape seed proanthocyanidin (GSP), a major polyphenol in grape seed, has several remarkable physiological activities beneficial to human health. However, the hydrophilicity of GSP hampers its cellular absorption and thus limits the bio-efficiency in vivo. In order to enhance the lipophilicity of GSP for improved bio-efficiency, lipophilic grape seed proanthocyanidin (LGSP) was synthesized by enzymatic esterification of GSP. The anti-inflammatory activity of LGSP was evaluated in lipopolysaccharides-stimulated RAW 264.7 cells in vitro and a zebrafish tail-amputated model in vivo. LGSP exhibited strong inhibitory effects on the production of NO and pro inflammatory cytokines TNF-alpha, IL-6 and IL-1 beta in RAW 264.7 cells. Further mechanistic studies showed that LGSP achieved the aforementioned effects by inhibiting the activation of MAPKs and NF-kappa B signaling pathways. In the zebrafish model, LGSP effectively suppressed the aggregation of neutrophils. Our findings suggest that LGSP may be effective as a complementary agent for the prevention/treatment of inflammation-mediated diseases.
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