期刊
ONCOTARGETS AND THERAPY
卷 14, 期 -, 页码 157-164出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/OTT.S278986
关键词
DDX11-AS1; miR-499b-5p; RWDD4; glioma
资金
- Zhejiang Provincial Natural Science Foundation [LGF18H090007]
- Basic Research Programs of Science and Technology Department of Wenzhou [Y2020219]
- Youth Talents Plan of Science and Technology Project of Zhejiang Provincial Health Commission [2019RC276]
This study found that upregulation of DDX11-AS1 in glioma is associated with poor prognosis, and it promotes glioma progression by interacting with miR-499b-5p to upregulate RWDD4.
Background: Long noncoding RNAs (IncRNA) exert essential functions during tumorigenesis. However, how lncRNAs participate in glioma development remains poorly researched. This study aimed to determine how DDX11-AS1 affects glioma progression. Methods: Gene expression was analyzed by qRT-PCR. Survival rate curve was plotted in 56 glioma patients. Loss-of-function assays were performed to analyze proliferation, migration, and invasion through CCK8, colony formation, and transwell assays. Luciferase assay and RNA pulldown assays were conducted to illustrate the underlying molecular mechanism. Results: DDX11-AS1 expression was upregulated in glioma tissues and cells. DDX11-AS1 overexpression was linked with poor prognostic value. DDX11-AS1 knockdown suppressed proliferation, migration, and invasion while inducing apoptosis. DDX11-AS1 interacted with miR-499b-5p to eliminate it, leading to upregulation of RWDD4 expression. RWDD4 was upregulated in glioma while miR-499b-5p was downregulated. Conclusion: DDX11-AS1 upregulation promotes glioma progression through acting as a competing endogenous RNA for miR-499b-5p to upregulate RWDD4.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据