期刊
VIRUS RESEARCH
卷 291, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.virusres.2020.198186
关键词
Influenza virus; H7N9; Adaptive mutation
类别
资金
- National Natural Science Foundation of China [81672005, U1611264, 81001271, 81721091]
- Mega-Project of National Science and Technologyfor the 12th and 13th Five-Year Plan of China [2018ZX10715-014-002, 2014ZX10004008]
The study identified an increased prevalence of the PB2-V292I mutation in H7N9 influenza viruses from both human and bird isolates over the past 6 years, with polymerase complexes containing PB2-292I showing increased activity. The PB2-740A mutation had a limited prevalence in 2013, and may have a negative effect on H7N9 virus replication in cells.
Characterization of host adaptation markers among human isolates is important for recognizing the potential for cross-species transmission in avian influenza A viruses. Here, we studied two new potential adaptive mutations, V292I and D740A, in the PB2 protein that were identified by a multi-factor regression model. The study shows that the prevalence of the PB2-V292I mutation is increased in H7N9 influenza viruses isolated from both humans and birds over the past 6 years. The phylogenetic tree showed that influenza A/H7N9 has a lineage based on the strains containing PB2-292I. Polymerase complexes containing PB2-292I/627K derived from H7N9 exhibit increased polymerase activity. PB2-292I coupled with 627K also enhances viral transcription and replication in cells, whereas PB2-292I alone did not show the same effect in the H7N9 virus. However, PB2-740A only had a limited prevalence in 2013, and the change from D to A in PB2-740A may have a negative effect on the replication of the H7N9 virus in cells.
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