期刊
VIROLOGY
卷 556, 期 -, 页码 1-8出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2021.01.011
关键词
Coronavirus; Porcine deltacoronavirus (PDCoV); NS6; Virions
类别
资金
- National Key Research and Development Program of China [2016YFD0500102]
- National Natural Science Foundation for Young Scientists of China [31802205]
The study found that the accessory protein NS6 is expressed during PDCoV infection in small intestinal tissues of piglets and is incorporated into virions. However, the anti-NS6 antibody was unable to neutralize PDCoV.
Porcine deltacoronavirus (PDCoV) is one of the emerged coronaviruses posing a significant threat to the swine industry. Previous work showed the presence of a viral accessory protein NS6 in PDCoV-infected cells. In this study, we detected the expression of the NS6 protein in small intestinal tissues of PDCoV-infected piglets. In addition, SDS-PAGE and Western blot analysis of sucrose gradient-purified virions showed the presence of a 13kDa NS6 protein. Further evidences of the presence of NS6 in the PDCoV virions were obtained by immunogold staining of purified virions with anti-NS6 antiserum, and by immunoprecipitation of NS6 from purified virions. Finally, the anti-NS6 antibody was not able to neutralize PDCoV in cultured cells. These data establish for the first time that the accessory protein NS6 is expressed during infection in vivo and incorporated into PDCoV virions.
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