Detection of coinfection of a divergent subgroup of genotype I Japanese encephalitis virus in multiple classical swine fever virus outbreaks in pigs of Assam, India

A retrospective investigation of pig tissue samples from different classical swine fever virus (CSFV) outbreaks was undertaken employing RT-PCR for possible coinfection with other swine viruses. Four samples from three different outbreaks were found to be coinfected with Japanese encephalitis virus (JEV). Phylogenetic analysis was done based on complete E gene sequenced from all four coinfected samples. This revealed a new introduction of a divergent subgroup of JEV genotype I in India. This is the first report of detection of coinfection of JEV and CSFV in pigs and the first incidence of JEV genotype I in pigs in India.

Automated summary

A retrospective investigation revealed the first incidence of co-infection of Japanese encephalitis virus (JEV) genotype I and CSFV in pigs in India, indicating a new introduction of a divergent subgroup of JEV genotype I. This study marks the first report of JEV genotype I in pigs in India, demonstrating a case of co-infection with CSFV.