期刊
TRANSBOUNDARY AND EMERGING DISEASES
卷 69, 期 2, 页码 278-285出版社
WILEY-HINDAWI
DOI: 10.1111/tbed.13976
关键词
cattle; cryptosporidiosis; Cryptosporidium parvum; diarrhoea; outbreak
资金
- Innovation Team Project of Guangdong University [2019KCXTD001]
- Higher Education Discipline Innovation Project [D20008]
- National Natural Science Foundation of China [31820103014, 32030109, U1901208]
The study showed a significantly higher infection rate of Cryptosporidium parvum during the outbreak, particularly associated with watery diarrhea in neonatal calves at a large cattle farm in Hebei Province, China. The subtype identified in the C. parvum isolates was IIdA20G1, highlighting the importance of monitoring and controlling the spread of this pathogen in China.
Cryptosporidium parvum is a major zoonotic pathogen responsible for outbreaks of severe diarrhoea in humans and calves. Almost all investigations of cryptosporidiosis outbreaks caused by C. parvum have focused on its IIa subtype family in industrialized nations. From December 2018 to April 2019, approximately 200 neonatal calves on a large cattle farm in Hebei Province, China, were diagnosed with watery diarrhoea and over 40 died. To investigate the cause of the outbreak, faecal samples were taken during and after the outbreak from neonatal calves of <= 4 weeks of age (n = 40 and n = 56) and older calves of 4-24 weeks of age (n = 79 and n = 38). A total of 18 faecal samples collected from ill calves at the peak of the outbreak were analysed for four common enteric pathogens using an enzymatic immunoassay (EIA). In addition, 75 samples from neonatal calves were tested for rotavirus by EIA. All samples were analysed for Cryptosporidium spp. using PCR and sequencing techniques. Of the initial 18 samples from sick calves, ten were positive for C. parvum, five for rotavirus, and one for coronavirus. The overall prevalence of rotavirus in neonatal calves was 20.0% (15/75), with no significant differences during and after the outbreak. In contrast, Cryptosporidium parvum infections were significantly higher during the outbreak (60.0%, 24/40) than after the outbreak (30.4%, 17/56; p = .004). Cryptosporidium parvum infection was associated with the presence of watery diarrhoea in neonatal calves (OR = 11.19), while no association was observed between C. bovis infection and diarrhoea. All C. parvum isolates were identified as subtype IIdA20G1. This is one of the few reports of outbreaks of severe diarrhoea caused by C. parvum IId subtypes in calves. More attention should be directed towards the dissemination of C. parvum in China.
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