期刊
TRANSBOUNDARY AND EMERGING DISEASES
卷 68, 期 4, 页码 2465-2476出版社
WILEY-HINDAWI
DOI: 10.1111/tbed.13914
关键词
Colombia; enteric swine disease; epidemiology of coronaviruses; molecular characterization of coronaviruses; Porcine Epidemic Diarrhea Virus; phylogenetics of coronaviruses; porcine epidemic diarrhea virus; recombination of coronaviruses
资金
- China Scholarship Council [201806760010]
- Asociacion Colombiana de Porciculotres - Porkcolombia
This study investigated the spread of Porcine Epidemic Diarrhea Virus (PEDV) in Colombia and found significant variations in the proportion of positive samples over time. Recombinant regions were identified in 21 strains of PEDV, with phylogenetic analysis revealing 7 lineages with common ancestors. Antigenic analysis showed residue differences in neutralizing epitopes in the spike and nucleocapsid proteins.
Porcine epidemic diarrhea virus (PEDV) is a significant global, enteric coronavirus in pigs and was first reported in Colombia in 2014. However, the epidemiology, genetic and antigenic characteristics of the virus have yet to be investigated. In this study, we investigated the dissemination of PEDV by testing 536 samples by RT-PCR over a 33-month period. The 35.8% of positive samples (n = 192) was significantly different (p < .01) between months over time, with a higher number of positives samples occurring at the beginning of the epidemic and during the second epidemic wave within the main pork producing region. The complete PEDV genomes were generated for 21 strains, which shared a high nucleotide and amino acid sequence identity, except for the spike (S) gene. Recombinant regions were identified within the Colombian strains and between Colombian and Asian PEDV strains. Phylogenetic analysis of the 21 Colombian strains demonstrated the presence of 7 lineages that shared common ancestors with PEDV strains from the United States. Moreover, the antigenic analysis demonstrated residue differences in the neutralizing epitopes in the spike and nucleocapsid proteins. Our results illustrated the simultaneous introduction of the two PEDV genotypes (GIIa American pandemic and S-INDEL) into the Colombian swine industry during the 2014 PEDV epidemic and enhanced our understanding of the epidemiology and molecular diversity of PEDV in Colombia.
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