Reproductive performance in gilts following applications of different insemination doses and techniques

This study evaluated the applicability of intrauterine artificial insemination (IUAI) in gilts and the impact of age at insemination and different body characteristics of gilts on the success rate for cannula insertion. Additionally, reproductive performance was evaluated for IUAI and cervical artificial insemination (CAI), considering different semen dose sizes. A total of 636 gilts were assigned in a 2 x 2 factorial design: two artificial insemination techniques (CAI and IUAI) and two semen dose sizes (1.5 x 10(9) sperm cells/50 mL or 2.5 x 10(9) sperm cells/80 mL). In those gilts assigned to IUAI (n = 319) the success rate for intrauterine cannula insertion was evaluated according to weight at first detected estrus, body condition score (BCS), and age at insemination. Reproductive performance, occurrence of bleeding, and semen backflow during all inseminations were compared among groups (2 x 2). Two subgroups were evaluated regarding the time expended to perform insemination (n = 380), and the semen backflow collected during 1 h after insemination (n = 114). The success rate for intrauterine cannula insertion, based on a successful insertion in all inseminations performed during estrus, was 58.9%. Additionally, greater possibility (>60%; P <= 0.04) of cannula insertion was observed in heavier gilts (>= 124 kg), as well as in older gilts (>= 225 d) and those with greater BCS (>3). There were no differences among the groups in pregnancy rate (>= 95.3%; P = 0.23), farrowing rate (>= 93.7%; P = 0.54), total piglets born (>= 14.5; P = 0.45), as well as, bleeding (P = 0.48) and backflow (P = 0.48) during insemination. However, the percentage of semen backflow volume and percentage of sperm cells in the backflow were lower in gilts inseminated by CAI with 1.5 billion sperm cells/50 mL (P < 0.01) than in the other groups. There was no expressive reduction in time expended to perform IUAI compared to CAI. However, gilts inseminated with 1.5 billion sperm cells/50 mL showed a lower total time to inseminate than all other groups (P < 0.01). In conclusion, higher weight, BCS, and age increased the success rate for cannula insertion. However, IUAI did not optimize the insemination procedure, and remains limited for gilts due to the low success rate for cannula insertion. Reproductive performance was not affected by IUAI or CAI using 1.5 or 2.5 billion sperm cells in 50 or 80 mL, respectively, suggesting the possibility of using CAI with 1.5 billion sperm cells/50 mL in gilts. (C) 2020 Elsevier Inc. All rights reserved.

Automated summary

This study evaluated the use of intrauterine artificial insemination (IUAI) in gilts and the factors affecting cannula insertion success. Heavier, older, and better body condition gilts had a greater success rate for cannula insertion. However, IUAI did not improve insemination procedure efficiency and was limited by low success rates for cannula insertion in gilts. Reproductive performance was not impacted by IUAI or cervical artificial insemination (CAI) using different semen dose sizes.