期刊
TALANTA
卷 221, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2020.121483
关键词
Surface plasmon resonance; Human plasma; Fouling; Betaine; Self-assembled monolayers; Disulfides
资金
- European Union's Horizon 2020 research and innovation programme [633937]
- MIUR Prin 2017 [2017RHX2E4]
- H2020 Societal Challenges Programme [633937] Funding Source: H2020 Societal Challenges Programme
Surface plasmon resonance (SPR) is widely used for detecting biomolecular systems, but faces challenges when analyzing blood plasma or serum samples due to non-specific adsorption. Traditional polymeric layers for antifouling may not be fully effective for SPR detection due to limitations in layer thickness. A new pentrimeric system is proposed for sensitive SPR detection with less than 2 nm thickness, showing potential for improving antifouling properties in molecular diagnostics.
Surface plasmon resonance (SPR) has been widely used to detect a variety of biomolecular systems, but only a small fraction of applications report on the analysis of patients' samples. A critical barrier to the full implementation of SPR technology in molecular diagnostics currently exists for its potential application to analyze blood plasma or serum samples. Such capability is mostly hindered by the non-specific adsorption of interfering species present in the biological sample at the functional interface of the biosensor, often referred to as fouling. Suitable polymeric layers having a thickness ranging from 15 and about 70 nm are usually deposited on the active surface of biosensors to introduce antifouling properties. A similar approach is not fully adequate for SPR detection where the exponential decay of the evanescent plasmonic field limits the thickness of the layer beyond the SPR metallic sensor surface for which a sensitive detection can be obtained. Here, a triethylene glycol (PEG (3))-pentrimer carboxybetaine system is proposed to fabricate a new surface coating bearing excellent antifouling properties with a thickness of less than 2 nm, thus compatible with sensitive SPR detection. The high variability of experimental conditions described in the literature for the quantitative assessment of the antifouling performances of surface layers moved us to compare the superior antifouling capacity of the new pentrimeric system with that of 4-aminophenylphosphorylcholine, PEG-carboxybetaine and sulfobetaine-modified surface layers, respectively, using undiluted and diluted pooled human plasma samples. The use of the new coating for the immunologic SPRI biosensing of human arginase 1 in plasma is also presented.
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