期刊
TALANTA
卷 220, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2020.121397
关键词
Au@AgNR; SERS nanotag; Antibiotic susceptibility testing; Minimum inhibitory concentration; Principal component analysis
资金
- National Natural Science Foundation of China [21605005, 81530030, 81873697, 21976209]
- National Research Foundation of Korea [2019R1A2C3004375, 2018M3A7B4071203]
- Research Initiation Fund of Binzhou Medical University [BY2019KYQD39]
- Taishan Scholar Project Special Funding [ts20190962]
There is a challenge to obtain an ultrasensitive and rapid approach to detect bacteria and identify resistance. As a powerful bioanalytical tool, surface-enhanced Raman scattering (SERS) in bacterial detection have attracted increasing attentions. Herein, we developed a SERS-active Au@Ag core-shell nanorod (Au@AgNR) tag platform for ultrasensitive bacteria detection and antibiotic-susceptibility testing (AST). The platform established that surface enhanced Raman scattered Rhodamine 6G (R6G) absorption at 1517 cm(-1) had a good linearity (RI = 3865 + 193logC; R-2 = 0.97) with logarithm of E. coli concentration over a range of 10(7)-10(2) CFU (colony forming unit)/mL with limit of detection as low 10(2) CFU/mL. When E. coli was exposed to ampicillin at minimum inhibitory concentration (MIC, 4 mu g/mL), Raman spectroscopy showed the obvious variation between ampicillin-susceptible E. coli (Amp(-)-E. coli) and the ampicillin-resistant E. coli (Amp(+)-E. coli). Combined with principal component analysis (PCA) statistical analysis, the Raman intensity variation mentioned above allows to obtain rapid antibiotic resistance testing (<3.5 h). In addition, E.coli spiked into blood from C57BL/6 mice can be identified clearly, indicating the potential for point-of-care diagnostics.
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