期刊
STROKE
卷 52, 期 1, 页码 260-270出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/STROKEAHA.120.032764
关键词
cerebrovascular circulation; endothelial cells; endothelium; vascular; models; theoretical; viruses
资金
- American Heart Association [20203858]
- University of California Los Angeles W.M. Keck Foundation COVID-19 Research Award Program
- Dr Miriam and Sheldon G. Adelson Medical Research Foundation
- NINDS [NS112799]
- NHLBI [HL118650, HL149808]
- Veterans Healthcare Administration [I01 BX004356]
This study discovered that brain endothelial cells are susceptible to SARS-CoV-2 infection due to flow-dependent expression of ACE2. The binding of viral S protein triggers a unique gene expression profile in brain endothelial cells, potentially explaining the association of SARS-CoV-2 infection with cerebrovascular events.
Background and Purpose: Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is associated with an increased rate of cerebrovascular events including ischemic stroke and intracerebral hemorrhage. The mechanisms underlying cerebral endothelial susceptibility and response to SARS-CoV-2 are unknown yet critical to understanding the association of SARS-CoV-2 infection with cerebrovascular events. Methods: Endothelial cells were isolated from human brain and analyzed by RNA sequencing. Human umbilical vein and human brain microvascular cells were used in both monolayer culture and endothelialized within a 3-dimensional printed vascular model of the middle cerebral artery. Gene expression levels were measured by quantitative polymerase chain reaction and direct RNA hybridization. Recombinant SARS-CoV-2 S protein and S protein-containing liposomes were used to measure endothelial binding by immunocytochemistry. Results: ACE2 (angiotensin-converting enzyme-2) mRNA levels were low in human brain and monolayer endothelial cell culture. Within the 3-dimensional printed vascular model, ACE2 gene expression and protein levels were progressively increased by vessel size and flow rates. SARS-CoV-2 S protein-containing liposomes were detected in human umbilical vein endothelial cells and human brain microvascular endothelial cells in 3-dimensional middle cerebral artery models but not in monolayer culture consistent with flow dependency of ACE2 expression. Binding of SARS-CoV-2 S protein triggered 83 unique genes in human brain endothelial cells including upregulation of complement component C3. Conclusions: Brain endothelial cells are susceptible to direct SARS-CoV-2 infection through flow-dependent expression of ACE2. Viral S protein binding triggers a unique gene expression profile in brain endothelia that may explain the association of SARS-CoV-2 infection with cerebrovascular events.
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