期刊
SMALL
卷 17, 期 3, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202005814
关键词
graphene; HOPG; near‐ field microscope; single biomolecules; terahertz
类别
资金
- National Key Research and Development Program of China [2017YFF0106303]
- National Natural Science Foundation of China [U1932132, 11604332]
- Natural Science Foundation of Chongqing [cstc2018jcyjAX0405, cstc2019jcyj-msxmX0654, cstc2019jcyj-msxmX0051]
- Shanghai Municipal Science and Technology Commission [19JC1410300]
- University of Chinese Academy of Sciences Supported Program for Tackling Key Problems in Science and Technology
A graphene-mediated THz scattering-type scanning near-field optical microscope is developed for direct imaging of single proteins, demonstrating that a graphene substrate with high THz reflectivity and atomic flatness provides high THz contrast against protein molecules. The use of an optimized length platinum probe enhances the amplitude of scattered THz near-field signals, allowing the topographical and THz scattering images of individual IgG and ferritin molecules with a few nanometers in size to be obtained simultaneously. This strategy opens up new avenues for imaging single biomolecules with THz.
Terahertz (THz) biological imaging has attracted intense attention due to its capability of acquiring physicochemical information in a label-free, noninvasive, and nonionizing manner. However, extending THz imaging to the single-molecule level remains a challenge, partly due to the weak THz reflectivity of biomolecules with low dielectric constants. Here, the development of graphene-mediated THz scattering-type scanning near-field optical microscope for direct imaging of single proteins is reported. Importantly, it is found that a graphene substrate with high THz reflectivity and atomic flatness can provide high THz contrast against the protein molecules. In addition, a platinum probe with an optimized shaft length is found enabling the enhancement of the amplitude of the scattered THz near-field signals. By coupling these effects, the topographical and THz scattering images of individual immunoglobulin G (IgG) and ferritin molecules with the size of a few nanometers are obtained, simultaneously. The demonstrated strategy thus opens new routes to imaging single biomolecules with THz.
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