期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 326, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.129007
关键词
Electrochemical sensors; Reactive oxygen and nitrogen species; Cold physical plasma; Toluidine blue; Hydrogen peroxide; In-situ detection
资金
- German Federal Ministry of Education and Research (BMBF) [03Z22DN12, 03Z22DN11]
The study presented the development of an electrochemical sensor for in-situ assessment of reactive species in redox-based therapies, enabling the detection of the production of reactive oxygen and nitrogen species like hydrogen peroxide through chronoamperometry at different potentials.
The extent of clinical applications of oxidative stress-based therapies such as photodynamic therapy (PDT) or respiratory chain disruptors are increasing rapidly, with cold physical plasma (CPP) emerging as a further option. According to the current knowledge, the biological effects of CPP base on reactive oxygen and nitrogen species (PONS) relevant in cell signaling. To monitor the safety and the biological impact of the CPP, determining the local generation of PONS in the same environment in which they are going to be applied is desirable. Here, for the first time, the development of an electrochemical sensor for the simple, quick, and parallel determination of plasma-generated reactive species is described. The proposed sensor consists of a toluidine blue redox system that is covalently attached to a gold electrode surface. By recording chronoamperometry at different potentials, it is possible to follow the in-situ production of the main long-lived reactive oxygen and nitrogen species like hydrogen peroxide, nitrite, hypochlorite, and chloramine with time. The applicability of this electrochemical sensor for the in-situ assessment of reactive species in redox-based therapies is demonstrated by the precise analysis of hydrogen peroxide dynamics in the presence of blood cancer cells.
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