期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 327, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128898
关键词
Electrochemical biosensing platform; Nanochannels; microRNA; G-quadruplex; Methylene blue
资金
- National Natural Science Foundation of China [21775120]
- Natural Science Foundation of Shaanxi Province of China [2018JM2017]
An ultrasensitive label-free electrochemical biosensing platform for detecting MiR-21 at a very low concentration was developed by immobilizing capture probes in nanochannels based on G-quadruplex formation. This platform was also successfully applied for MiR-21 detection in serum samples, and has the potential for the assay of other scarce tumor-related genes.
Ultrasensitive detection of microRNA is crucial important in the diagnosis of related diseases. By immobilizing the capture probes in nanochannels, we developed a label-free electrochemical biosensing platform based on guanine-quadruplex (G-quadruplex) formation in nanochannels. The designed capture probe contains both the microRNA-21 (MiR-21) recognition sequence and guanine-rich sequence. The presence of the target MiR-21 would trigger the enzymatic cleaving of the RNA/DNA heteroduplexes which led some of the guanine-rich sequences escape from the nanochannels and thus reduced the formation of the G-quadruplex/hemin complex. Therefore, the quantity of methylene blue fluxed through the nanochannels changed because of the variation of the steric hindrance. A optimized carbon nanofibers modified electrode was applied to monitor the quantity difference of methylene blue. In this way, the biosensing platform realized the label-free electrochemical detection of MiR-21 with a ultralow detection limit of 0.5 aM. Meanwhile, this biosensing platform was assessed for MiR-21 detection in serum samples. The biosensing platform can be readily extended to the assay of other scarcetumor-related genes.
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