期刊
SCIENTIA HORTICULTURAE
卷 276, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scienta.2020.109706
关键词
Broccoli sprouts; Mannose treatment; gamma-aminobutyric acid (GABA); Health-promoting metabolites
类别
资金
- Natural Science Foundation of China [31972092, 31772030]
- Public Welfare Applied Research Project of Zhejiang Province [LGN19C200012]
- Ningbo City [2019C10086]
- Key Research and Development Projects of Zhejiang Province [2017C02021]
- Scientific Research Foundation of Graduate School of Ningbo University [G19032]
Mannose treatment significantly enhances the content of GABA in germinated broccoli sprouts and promotes the synthesis of other beneficial health-promoting metabolites, such as anthocyanins and total glucosinolates.
The contents of anthocyanins, total glucosinolates, sulforaphane, and the metabolism of gamma-aminobutyric acid (GABA) in broccoli sprouts grown under 1.0 mmol L-1 of mannose were investigated. The results showed that mannose treatment improved glutamate (Glu) content and the activity of diamine oxidase (DAO), and reduced gamma-aminobutyric acid transaminase (GABA-T) activity, which enriched GABA biosynthesis. Mannose treatment enhanced the activities of diamine oxidase (DAO), polyamine oxidase (PAO), and 4-aminobutyraldehyde dehydrogenase (ABALDH), and increased the conversion rate of free polyamine. At the same time, it is also beneficial for the enrichment of anthocyanins, total glucosinolates and sulforaphane. These results indicated that mannose treatment has a significant enhancement effect on GABA and other health-promoting metabolites in germinated broccoli.
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