A multibiomarker approach in the caged neotropical fish to assess the environment health in a river of central Brazilian Cerrado

Water safety is a world-wide concern and several efforts have been made in order to ensure the conservation of aquatic ecosystems. Water quality monitoring must be performed with an integrated approach using biomonitor organisms allied to water parameters. Nonetheless, very few studies have focused on biomarker responses in neotropical fish, especially in the freshwater ecosystem of Brazilian Cerrado savanna. In present study, the active biomonitoring of the Joao Leite river (central Brazilian Cerrado river) was performed through the evaluation of biomarker responses in caged Astyanax lacustris in combination with land use classification and analysis of water parameters. Caged fish were exposed for seven days at four sites along the river and two control groups were kept in a tank under controlled conditions. Results showed that pasture was the predominant land use in the Joao Leite river basin (54.07%), followed by natural vegetation (34.92%) and other kind of land use (11.01%). Water analyses showed metal concentrations (Mn and Fe) above the maximum allowed by Brazilian regulation, with particularly higher concentrations at Site 2 (near to pasture area). Biomarker responses did not show significant differences for somatic and mutagenic biomarkers between sites. However, the comet assay showed high DNA damage at Sites 2 and 3, indicating genotoxic effects in caged fish at pasture areas. Histopathological analysis showed highest frequency of leukocyte infiltration in liver of fish from Site 2, confirming the ecotoxic effects on A. lacustris in streams impacted by grazing activities. DNA damage and leukocyte infiltration in fish hepatic tissues were sensitive biomarkers in the neotropical fish A. lacustris to assess the environment health of the Cerrado river. These results showed the importance of using a multibiomarker approach in environmental risk assessment, especially in areas more at risk from anthropogenic pollution. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

Water safety is a global concern, with efforts being made to protect aquatic ecosystems. In this study, biomarker responses in caged Astyanax lacustris were evaluated in the Joao Leite river, central Brazilian Cerrado, along with land use classification and water parameter analysis. Results revealed high metal concentrations in the water, particularly near pasture areas, leading to genotoxic effects in caged fish. This study highlights the importance of using a multi-biomarker approach for environmental risk assessment in areas at higher risk of anthropogenic pollution.