Physiological and metabolomics responses of two wheat (Triticum aestivum L.) genotypes differing in grain cadmium accumulation

To reduce cadmium (Cd) pollution of food chains, screening and breeding of low-Cd-accumulating genotypes have received increasing attention. However, the mechanisms involving Cd tolerance and accumulation are not fully understood. Here, we investigated the physiological responses and metabolomics profiling on two wheat (Triticum aestivum L) genotypes, a low-Cd-accumulating genotype in grains (Aikang58, AK58) and a high-Cd-accumulating genotype in grains (Zhenmai10, ZM10), in hydroponic culture treated without/with Cd for 7 days. The results showed that AK58 was a Cd tolerant genotype with higher capacity of antioxidant systems in root. In addition, the concentrations of Cd bound to root cell walls were higher in AK58 than ZM10, of which pectin and hemicellulose played important roles in Cd binding. Moreover, subcellular distribution manifested that Cd sequestrated in the vacuoles was another tolerance mechanism in AK58. Simultaneously, metabolomics profiling showed that, in AK58, phenylalanine metabolism, alanine, aspartate and glutamate metabolism, isoquinoline alkaloid biosynthesis, arginine and proline metabolism, arginine biosynthesis and glyoxylate and dicarboxylate metabolism are highly related to antioxidant defense system, cell wall biosynthesis and metabolisms of phytochelatins together with other organic ligands, playing crucial roles in Cd tolerance and Cd fixation mechanisms in roots. These novel findings should be useful for moleadar assisted screening and breeding of low Cd-accumulating genotypes for wheat crop. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

The study revealed that the low-Cd-accumulating wheat genotype, AK58, exhibited stronger Cd tolerance and higher antioxidant system capacity in roots compared to the high-Cd-accumulating genotype, ZM10. Additionally, AK58 had higher concentrations of Cd bound to root cell walls, with pectin and hemicellulose playing important roles in Cd binding. Moreover, Cd sequestration in vacuoles was identified as another tolerance mechanism in AK58.