期刊
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
卷 56, 期 1, 页码 20-29出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00365521.2020.1844793
关键词
Crohn’ s disease; vitamin D; tight junction; Treg; Th17
资金
- National Natural Science Foundation of China [81770553]
- key project of a science and technology development fund from Nanjing Medical University [2017NJMUCX003]
- Postgraduate Research AMP
- Practice Innovation Program of Jiangsu Province [SJCX19_0317]
Vitamin D deficiency is common in Crohn's disease patients, and is associated with disease activity and intestinal inflammation, potentially affecting the balance of Treg/Th17 cells and the expression of gut TJ proteins.
Background High prevalence of vitamin D deficiency has been found among Crohn's disease (CD) patients. Vitamin D probably participates in the pathogenesis of CD, but this idea remains controversial. This study was to investigate the levels of vitamin D in CD patients and analyze the relationship between vitamin D and intestinal inflammation. Methods Vitamin D levels were measured by chemiluminescence immunoassay in 198 CD patients (96 in active, 102 in remission) and 100 healthy controls. The correlation between vitamin D levels and clinical parameters was analysed. The expression of intestinal tight junction (TJ) proteins in CD patients was measured by immunofluorescence staining. Treg and Th17 percentages in the peripheral blood were determined by flow cytometry. Results CD patients exhibited significantly lower 25(OH)D levels than healthy controls, especially in active CD patients. Serum 25(OH)D levels in CD patients were negatively correlated with the CD activity index (CDAI), the simple endoscopic score for CD (SES-CD), and inflammatory markers, including erythrocyte sedimentation rate (ESR), platelet (PLT) count and faecal calprotectin (FC) levels. Moreover, in patients with vitamin D deficiency, the expression of TJ proteins (Occludin, claudin-1, ZO-1 and JAM-1) in the intestinal mucosa was reduced, and Treg cells in the peripheral blood were decreased, while Th17 cells were increased compared to those with vitamin D sufficiency and controls. Conclusions Vitamin D deficiency in CD patients is common. Vitamin D is associated with disease activity and intestinal inflammation, which may affect the Treg/Th17 balance and the expression of gut TJ proteins.
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