期刊
DYES AND PIGMENTS
卷 132, 期 -, 页码 7-19出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2016.03.054
关键词
Stacking; Stability; Conjugation; Fluorescence; Image guided surgery
资金
- European Research Council under the European Union's Seventh Framework Program (FP7)
- ERC [2012-306890, 2012-323105]
- Dutch VIDI grant of the Organization for Scientific Research [STW BGT 11272]
- Dutch Cancer Society [PGF 2009-4344]
The most commonly used near-infrared cyanine dyes contain an aryl ether that is not fully stable towards nucleophiles. Replacement of the aryl ether by a more stable carbon-carbon bond can improve the stability. In this work we have synthesized a series of four negatively-charged symmetrical C-C bond containing Cy7 derivatives and compared them to the known dyes indocyanine green (ICG) and IRDye 800CW. The extent of stacking of these C-C bond-containing dyes was higher than reported for aryl ether dyes, but stacking could be minimized by altering the surface charge of the molecules and by introducing sulfonate groups. Furthermore, the degree of stacked dye in an antibody-dye conjugate was similar to the degree of stacking of free dye under labeling conditions. In our view, C-C bond-containing Cy7 dyes provide a chemical platform, based on which one can improve the photophysical properties and stacking behavior, thereby generating interesting additions to the conjugation toolbox available for e.g. antibodies. (C) 2016 Elsevier Ltd. All rights reserved.
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