Specific ligands based on Troger's base derivatives for the recognition of glycosaminoglycans

Symmetric Trager's base derivatives with quinolinium substitution, 7 and 8, were prepared, and their structures were confirmed by H-1 and C-13 NMR, 2D NMR and NOE NMR techniques and elemental analysis. These compounds were studied as specific ligands for the recognition of glycosaminoglycans (heparin, heparan sulphate, chondroitin sulphate and hyaluronic acid). They exhibited high affinity and selectivity towards glycosaminoglycans, especially heparin. The conditional binding constants, linear ranges and detection limits were determined by UV-Vis spectroscopy. These values, especially at extremely low limits of detection, showed the high potential of the prepared ligands for the recognition and determination of glycosaminoglycans, especially heparin. For example, the minimum detectable heparin concentrations for 7 and 8 were 0.1 and 0.17 ng/mL (R-2 = 0.988 and 0.9938) in media (1 mM NaCl (H2O:MeOH; 7:3, v/v), pH = 7.34), respectively. (C) 2016 Elsevier Ltd. All rights reserved.