期刊
RNA BIOLOGY
卷 18, 期 11, 页码 1778-1790出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2020.1870844
关键词
Bacteriophage; gene expression; transcriptome; RNA-seq; P. aeruginosa
资金
- Portuguese Foundation for Science and Technology (FCT) [PTDC/BBB-BSS/6471/2014, POCI-01-0145-FEDER-016643, UIDB/04469/2020, SFRH/BD/133193/2017]
- European Research Council under the European Union's Horizon 2020 research and innovation programme [819800]
- European Research Council (ERC) [819800] Funding Source: European Research Council (ERC)
- Fundação para a Ciência e a Tecnologia [SFRH/BD/133193/2017, PTDC/BBB-BSS/6471/2014] Funding Source: FCT
RNA sequencing of phage-infected bacterial cultures provides insights into the transcriptional events of bacterial response to phage infection. Regardless of the growth medium, phage LUZ19 induces significant transcriptional changes in the bacterial host, particularly during early infection stages.
RNA sequencing of phage-infected bacterial cultures offers a snapshot of transcriptional events occurring during the infection process, providing insights into the phage transcriptional organization as well as the bacterial response. To better mimic real environmental contexts, we performed RNA-seq of Pseudomonas aeruginosa PAO1 cultures infected with phage LUZ19 in a mammalian cell culture medium to better simulate a phage therapy event and the data were compared to lysogeny broth medium. Regardless of the media, phage LUZ19 induces significant transcriptional changes in the bacterial host over time, particularly during early infection (t = 5 min) and gradually shuts down bacterial transcription. In a common response in both media, 56 P. aeruginosa PAO1 genes are differentially transcribed and clustered into several functional categories such as metabolism, translation and transcription. Our data allowed us to tease apart a medium-specific response during infection from the identified infection-associated responses. This reinforces the concept that phages overtake bacterial transcriptome in a strict manner to gain control of the bacterial machinery and reallocate resources for infection, in this case overcoming the nutritional limitations of the mammalian cell culture medium. From a phage therapy perspective, this study contributes towards a better understanding of phage-host interaction in human physiological conditions and demonstrates the versatility of phage LUZ19 to adapt to different environments.
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