期刊
DYES AND PIGMENTS
卷 133, 期 -, 页码 372-379出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2016.06.022
关键词
Intracellular pH probe; Lysosome imaging; Glycosylation; Naphthalimide; Photo-induced electron transfer
资金
- 973 project [2013CB733700]
- National Natural Science Foundation of China [21402050, 21420102004]
- Programme of Introducing Talents of Discipline to Universities [B16017]
- Shanghai Rising-Star Program [16QA1401400]
A series of galactosyl naphthalimide-piperazine derivatives have been synthesized as intracellular pH and lysosome-targeting imaging probes for live human hepatoma cells. The probes show good sensitivity in both aqueous buffer and intracellular environments. Incorporation of the galactose moiety with the pH probes facilitates their specific endocytosis and, thus targeted trafficking to lysosome, by the asialoglycoprotein receptor of human hepatoma cells. The acidic intracellular pH of live human hepatoma cells gives rise to a fluorescence turn-on signal of the probes probably via a modulation of the photo induced electron transfer (PET) mechanism. Additionally, galactosylation of the probes enhances their selective accumulation in lysosome and decreases the cytotoxicity. (C) 2016 Elsevier Ltd. All rights reserved.
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