期刊
REPRODUCTIVE SCIENCES
卷 28, 期 7, 页码 1910-1921出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s43032-020-00429-7
关键词
PTEN; Granulosa cells; Gene expression; Ovarian response; Transfection
资金
- National Natural Science Foundation of China [81972714]
- Natural Science Foundation of Fujian Province of China [2019 J01140, 2018 J01148]
- Young and Middle-aged Talent Training Program of Fujian Province of China [2019-ZQN-91]
- Science and Technology Project of Quanzhou [(2004)0010]
The ovarian reserve, controlled by PTEN gene expression, is crucial for the success of IVF and embryo transfer treatment. Interestingly, PTEN levels were higher in poor responders and lower in high responders, indicating its role in ovarian response. Additionally, PTEN may be involved in follicular dysplasia and ovarian dysfunction, suggesting its importance in regulating follicular development.
The ovarian reserve determines the success of in vitro fertilization (IVF) and embryo transfer treatment. It predicts the ovarian response in controlled ovarian hyperstimulation cycles. Apoptosis in granulosa cells surrounding oocytes is important for ovarian function and has been closely associated with follicular atresia. PTEN (encoding phosphatase and tensin homolog) is a well-known tumor suppressor gene that functions as a mediator of apoptosis and is crucial for mammal reproduction. In the present study, we analyzed the expression level of PTEN in human granulosa cells and aimed to investigate its association with the ovarian response and clinical outcomes in IVF. Apoptosis in granulosa cells were analyzed using Annexin V-Allophycocyanin staining after PTEN short hairpin RNA lentivirus transfection. Real-time fluorescent quantitative PCR analysis showed that the PTEN transcript level was significantly higher in poor responders and significantly lower in high responders, compared with that in normal responders. However, PTEN expression in the pregnancy group decreased slightly, but not significantly, compared with that in the non-pregnancy group. The apoptosis rate of granulosa cells declined significantly after 24-h transfection of the PTEN-shRNA lentivirus. These results suggest a fundamental role of PTEN in the regulation of follicular development, and that it might be involved in the pathogenesis of follicular dysplasia and ovarian dysfunction.
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