Regulation of the microsomal proteome by salicylic acid and deficiency of phosphatidylinositol-4-kinases β1 and β2 in Arabidopsis thaliana

Phosphatidylinositol-4-kinases beta 1 and beta 2 (PI4K beta 1/PI4K beta 2), which are responsible for phosphorylation of phosphatidylinositol to phosphatidylinositol-4-phosphate, have important roles in plant vesicular trafficking. Moreover, PI4K beta 1/PI4K beta 2 negatively regulates biosynthesis of phytohormone salicylic acid (SA), a key player in plant immune responses. The study focused on the effect of PI4K beta 1/PI4K beta 2 deficiency and SA level on the proteome of microsomal fraction. For that purpose we used four Arabidopsis thaliana genotypes: wild type; double mutant with impaired function of PI4K beta 1/PI4K beta 2 (pi4k beta 1/pi4k beta 2) exhibiting high SA level; sid2 mutant with impaired SA biosynthesis depending on the isochorismate synthase 1 and triple mutant sid2/pi4k beta 1/pi4k beta 2. We identified 1797 proteins whose levels were changed between genotypes. We showed that increased SA concentration affected the levels of 473 proteins. This includes typical SA pathway markers but also points to connections between SA pathway and clathrin-independent endocytosis (flotillins) and exocytosis/protein secretion (syntaxins, tetraspanin) to be investigated in future. In contrast to SA, the absence of PI4K beta 1/PI4K beta 2 itself affected only 27 proteins. Among them we identified CERK1, a receptor for chitin. Although PI4K beta 1/PI4K beta 2 deficiency itself did not have a substantial impact on the proteome of the microsomal fraction, our data clearly show that it enhances proteome changes when SA pathway is modulated in parallel.

Automated summary

The study investigated the impact of PI4K beta 1/PI4K beta 2 deficiency and SA level on the proteome of plant microsomes, identifying changes in protein levels between genotypes. The results indicate that increased SA concentration affects the levels of specific proteins and suggests potential connections between the SA pathway and clathrin-independent endocytosis/exocytosis. Additionally, while the absence of PI4K beta 1/PI4K beta 2 itself had minimal effect on the microsomal proteome, it enhanced proteome changes when the SA pathway was modulated.