期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2014442118
关键词
amyloid; cross-alpha; functional fibril; antimicrobial peptides
资金
- Israel Science Foundation [560/16]
- Israel Ministry of Science, Technology Space [78567]
- U.S.-Israel Binational Science Foundation [2017280]
- BioStruct-X - Seventh Framework Programme (FP7)
- Infrastructure for NMR, EM and X-rays for Translational Research (iNEXT) consortium of the European research infrastructure in structural biology (Instruct-ERIC)
- Direct For Social, Behav & Economic Scie
- Division Of Behavioral and Cognitive Sci [2017280] Funding Source: National Science Foundation
The study found that two subtypes of antimicrobial peptide uperin 3.5 can form functional amyloid fibrils, which has important physiological implications. Uperin 3.5 mainly forms cross-beta fibrils in the absence of lipids, but helical fibril formation is induced by bacterial cells or membrane mimetics, leading to membrane damage and cell death.
Antimicrobial activity is being increasingly linked to amyloid fibril formation, suggesting physiological roles for some human amyloids, which have historically been viewed as strictly pathological agents. This work reports on formation of functional cross-a amyloid fibrils of the amphibian antimicrobial peptide uperin 3.5 at atomic resolution, an architecture initially discovered in the bacterial PSM alpha 3 cytotoxin. The fibrils of uperin 3.5 and PSMa3 comprised antiparallel and parallel helical sheets, respectively, recapitulating properties of beta-sheets. Uperin 3.5 demonstrated chameleon properties of a secondary structure switch, forming mostly cross-beta fibrils in the absence of lipids. Uperin 3.5 helical fibril formation was largely induced by, and formed on, bacterial cells or membrane mimetics, and led to membrane damage and cell death. These findings suggest a regulation mechanism, which includes storage of inactive peptides as well as environmentally induced activation of uperin 3.5, via chameleon cross-alpha/beta amyloid fibrils.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据