期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2021190118
关键词
TCR; T cell receptor; T cells; single-cell sequencing; mRNA sequencing
资金
- National Cancer Institute [U01 CA233074]
- Parker Institute for Cancer Immunotherapy [20163828]
- UCLA Broad Stem Cell Research Center (BSCRC)
- UCLA Tumor Immunology Training Grant (US Department of Health and Human Services Ruth L. Kirschstein Institutional National Research Service Award) [T32 CA009056]
- UCLA BSCRC predoctoral fellowship
- NIH [5P30 AI028697]
This study introduces a method called CLInt-Seq, which utilizes mRNA sequencing to efficiently recover antigen-specific TCRs from cells stained for specific intracellular proteins. This technique enables high-throughput identification and isolation of TCRs with low frequencies, as well as profiling of regulatory T cells and other cell phenotypes based on intracellular markers.
T cell receptors (TCRs) are generated by somatic recombination of V/D/J segments to produce up to 10(15) unique sequences. Highly sensitive and specific techniques are required to isolate and identify the rare TCR sequences that respond to antigens of interest. Here, we describe the use of mRNA sequencing via cross-linker regulated intracellular phenotype (CLInt-Seq) for efficient recovery of antigen-specific TCRs in cells stained for combinations of intracellular proteins such as cytokines or transcription factors. This method enables high-throughput identification and isolation of low-frequency TCRs specific for any antigen. As a proof of principle, intracellular staining for TNF alpha and IFN gamma identified cytomegalovirus (CMV)- and Epstein-Barr virus (EBV)-reactive TCRs with efficiencies similar to state-of-the-art peptide-MHC multimer methodology. In a separate experiment, regulatory T cells were profiled based on intracellular FOXP3 staining, demonstrating the ability to examine phenotypes based on transcription factors. We further optimized the intracellular staining conditions to use a chemically cleavable primary amine cross-linker compatible with current single-cell sequencing technology. CLInt-Seq for TNF alpha and IFN gamma performed similarly to isolation with multimer staining for EBV-reactive TCRs. We anticipate CLInt-Seq will enable droplet-based single-cell mRNA analysis from any tissue where minor populations need to be isolated by intracellular markers.
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