期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2014562118
关键词
follicular helper T cells; transcriptional regulation; coinhibitory pathway
资金
- NIH [AI112579, AI121080, AI139874, P30 CA16056-42]
- Veteran Affairs Biomedical Laboratory Research and Development Merit Review Program [BX002903]
- National Natural Science Foundation of China [31801222, 32070888, 82022031]
Precise regulation of coinhibitory receptors is crucial for maintaining immune tolerance and protective immunity. In protein immunization, T-FH cells lacking Tcf1 and Lef1 show abnormal upregulation of CTLA4 and LAG3, impacting B-cell help. Targeting CTLA4 and LAG3 can rectify T-FH cell defects and balance immune responses.
Precise regulation of coinhibitory receptors is essential for maintaining immune tolerance without interfering with protective immunity, yet the mechanism underlying such a balanced act remains poorly understood. In response to protein immunization, T follicular helper (T-FH) cells lacking Tcf1 and Lef1 transcription factors were phenotypically normal but failed to promote germinal center formation and antibody production. Transcriptomic profiling revealed that Tcf1/Lef1-deficient T-FH cells aberrantly upregulated CTLA4 and LAG3 expression, and treatment with anti-CTLA4 alone or combined with anti-LAG3 substantially rectified B-cell help defects by Tcf1/Lef1-deficient T-FH cells. Mechanistically, Tcf1 and Lef1 restrain chromatin accessibility at the Ctla4 and Lag3 loci. Groucho/Tle corepressors, which are known to cooperate with Tcf/Lef factors, were essential for T-FH cell expansion but dispensable for repressing coinhibitory receptors. In contrast, mutating key amino acids in histone deacetylase (HDAC) domain in Tcf1 resulted in CTLA4 derepression in T-FH cells. These findings demonstrate that Tcf1-instrinsic HDAC activity is necessary for preventing excessive CTLA4 induction in protein immunization-elicited T-FH cells and hence guarding their B-cell help function.
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