期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2005134118
关键词
protein secretion; biotinylation; mouse model
资金
- NIA NIH HHS [P30 AG024827] Funding Source: Medline
This study utilizes proximity biotinylation to elucidate tissue-specific secretome in vivo, identifying endothelial and muscle-derived serum proteins. The genetic platform is valuable for understanding normal and disease physiology, and designing tissue-specific disease biomarkers.
At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should, therefore, be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers.
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