期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2010140118
关键词
JNK; microtubule stability; cell death; Drosophila development; NAT (N-terminal acetyltransferase)
资金
- National Research Foundation of Korea - Ministry of Education, Science and Technology, Republic of Korea [NRF-2014K1A1A2042982, NRF-2017R1A2B3007516]
The N-terminal acetyltransferase Mnat9 plays a crucial role in regulating cell signaling and microtubule stability in Drosophila, with its loss causing severe developmental defects. Mnat9 is associated with mitotic spindles, enhances microtubule stability, and protects microtubules from severing protein Spastin. Additionally, it is functionally conserved across species, as human NAT9 can rescue Mnat9 RNAi phenotypes in flies.
Regulation of microtubule stability is crucial for the maintenance of cell structure and function. While the acetylation of alpha-tubulin lysine 40 by acetylase has been implicated in the regulation of microtubule stability, the in vivo functions of N-terminal acetyltransferases (NATs) involved in the acetylation of N-terminal amino acids are not well known. Here, we identify an N-terminal acetyltransferase, Mnat9, that regulates cell signaling and microtubule stability in Drosophila. Loss of Mnat9 causes severe developmental defects in multiple tissues. In the wing imaginal disc, Mnat9 RNAi leads to the ectopic activation of c-Jun N-terminal kinase (JNK) signaling and apoptotic cell death. These defects are suppressed by reducing the level of JNK signaling. Overexpression of Mnat9 can also inhibit JNK signaling. Mnat9 colocalizes with mitotic spindles, and its loss results in various spindle defects during mitosis in the syncytial embryo. Furthermore, overexpression of Mnat9 enhances microtubule stability. Mnat9 is physically associated with microtubules and shows a catalytic activity in acetylating N-terminal peptides of aand (3-tubulin in vitro. Cell death and tissue loss in Mnat9-depleted wing discs are restored by reducing the severing protein Spastin, suggesting that Mnat9 protects microtubules from its severing activity. Remarkably, Mnat9 mutated in the acetyl-CoA binding site is as functional as its wild-type form. We also find that human NAT9 can rescue Mnat9 RNAi phenotypes in flies, indicating their functional conservation. Taken together, we propose that Mnat9 is required for microtubule stability and regulation of JNK signaling to promote cell survival in developing Drosophila organs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据