期刊
POULTRY SCIENCE
卷 100, 期 4, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.psj.2021.01.005
关键词
DFM; probiotic; Bacillus; Clostridium perfringens; necrotic enteritis
资金
- Chr. Hansen A/S, Animal Health Innovation, Hoersholm, Denmark
- Chr. Hansen
The study evaluated the probiotic effects of 3 different Bacillus strains and their combination on broiler chickens through in vitro and in vivo experiments. The results showed that the multi-strain combination significantly improved the performance and reduced the necrotic enteritis lesion scores of the chickens, as well as decreased the mortality rate caused by necrotic enteritis.
The application of probiotics in broiler feed, to alleviate performance deficiencies due to mild infections by coccidia and Clostridium perfringens, is of increasing interest for the poultry industry. Therefore, our objective was to evaluate the capacity of 3 Bacillus strains and their combination as probiotics in vitro and in vivo. Thus, protein and carbohydrate degradation and C. perfringens growth inhibition capabilities were assessed by colometry measurement and an agar diffusion bioassay, respectively. A total of 2,250 1-day-old male broiler chicks were assigned to 5 dietary treatments: 1) non-probiotic-supplemented control (control); 2) control 1 DSM 32324 at 0.8 x 10(6) cfu/g of feed; 3) control 1 DSM 32325 at 0.5 x 10(6) cfu/g of feed; 4) control 1 DSM 25840 at 0.3 x 10(6) cfu/g of feed; and 5) control 1 DSM 32324 1 DSM 32325 1 DSM 25840 at 1.6 x 10(6) cfu/g of feed. A pathogenic field strain of C. perfringens was used to induce the necrotic enteritis challenge on day 19, 20, and 21. All birds and remaining feed were weighed on pen basis on day 0, 21, 35, and 42, to calculate BW gain and mortality-adjusted feed conversion. Mortality and mortality due to necrotic enteritis were recorded daily. On day 21, 45 birds per treatment were evaluated for macroscopic intestinal necrotic enteritis lesions. Performance data were statistically analyzed using an ANOVA and subjected to a least significant difference comparison. Necrotic enteritis lesion scores were statistically analyzed using non-parametric Kruskal-Wallis test. Dunn's test was used for treatment comparison. The tested strains showed different abilities of degrading protein and carbohydrates and inhibiting C. perfringens growth in vitro. The birds fed the multi-train combination presented significantly better performance and lower necrotic enteritis lesion score than those in the control group. Dietary supplementation with probiotics resulted in significantly lower necrotic enteritis mortality. The results demonstrate the suitability of the evaluated Bacillus multistrain combination as an effective probiotic in C. perfringens-challenged chickens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据