期刊
POULTRY SCIENCE
卷 100, 期 4, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.psj.2021.01.009
关键词
infectious laryngotracheitis virus; fowl pox virus; fowl pox virus integrated; reticuloendotheliosis virus; multiplex PCR
资金
- Animal and Plant Quarantine Agency (APQA), Ministry of Agriculture, Food and Rural Affairs, the Republic of Korea [B-1543084-2019-20-01]
The study developed a multiplex PCR assay for the simultaneous detection of ILTV, FWPV, REV-integrated FWPV, and REV, with a sensitivity of 1 x 54 copies/tube. The multiplex PCR showed 100% agreement with singleplex PCRs and sequencing when testing clinical samples, providing a simple, rapid, sensitive, specific, and cost-effective method for detecting the 4 viruses in clinical specimens.
Infectious laryngotracheitis (ILT), fowlpox (FP), and reticuloendotheliosis are important poultry diseases caused by gallid herpesvirus 1 (ILTV), fowlpox virus (FWPV), and reticuloendotheliosis virus (REV), respectively. Coinfections with ILTV and FWPV occur naturally in chickens, and FP in its more virulent wet form is characterized by diphtheritic lesions and easily confused with ILT. Moreover, the insertion of only partial REV-LTR or a nearly full-length REV into the FWPV genome, located between the ORF 201 and ORF 203, has increased recently in wild-type field FWPV isolates. Therefore, it is critical to detect ILTV, FWPV, REV-integrated FWPV, and REV early and accurately. In this study, we successfully developed a multiplex PCR assay for the simultaneous detection of ILTV, FWPV, REV-integrated FWPV, and REV, and the detection limits was 1 x 54 copies/tube. When used to test clinical samples, the results of the multiplex PCR were in 100% agreement with singleplex PCRs and sequencing. This new multiplex PCR is a simple, rapid, sensitive, specific, and cost-effective method for detection of 4 viruses in clinical specimens.
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