期刊
POLISH JOURNAL OF MICROBIOLOGY
卷 69, 期 4, 页码 471-478出版社
POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS
DOI: 10.33073/pjm-2020-051
关键词
porcine astroviruses; viral metagenomics; genome recombination
类别
资金
- National Key Research and Development Programs of China [2017YFC1200201]
- Jiangsu Provincial Key Research and Development Projects [BE2017693]
- Independent Project of Chengdu Research Base of Giant Panda Breeding [2020CPB-C11]
Porcine astroviruses (PAstVs) have wide distribution in swine herds worldwide. At present, five porcine astrovirus genotypes have been identified. In this study, using viral metagenomics, a novel PAstV strain (designated as Ahast) was identified in fecal samples from pigs in Anhui of China, and the complete genomic sequence of Ahast was obtained by assembling and PCR amplification. Genomic structural analysis indicated that Ahast had a typical ribosomal frameshifting signal, and some conserve amino acid motifs were also found in virally encoded proteins. Phylogenetic analysis and sequence comparison indicated that this virus belonged to porcine astrovirus genotype 4 (PAstV4), which formed a Glade dustered with other PAstV4. Multiple recombinant events were confirmed by recombination analysis and indicated that Ahast was a potential recombinant. Epidemiological investigation indicated that PAstV4 has a 10.7% prevalence in this pig farm. The new recombinant identified in this study will be beneficial to comprehend the origin, genetic diversity, and evolution of porcine astroviruses in Anhui of China.
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